生物活性
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.
细胞效力(Cellular Effect)
Cell Line
Type
Value
Description
References
HEK293
IC50
1.1 μM
Compound: propidium iodide
Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
[PMID: 23984975]
体外研究(In Vitro)
使用方法
1.1 制备储存液
用 DDH2O 配制 1 mg/mL 储备液。
1.2 工作液的配制
用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 20-50 μg/mL 的 Propidium Iodide 工作液。
注：请根据实际情况调整 Propidium Iodide 工作液浓度，且现用现配。
2. 细胞染色（悬浮细胞）
2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL。
2.2 加入 1 mL 工作液，室温孵育 5-10 分钟。
2.3 400 g，离心 3-4 分钟，弃去上清。
2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。
2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。
3. 细胞染色（贴壁细胞）
3.1 将贴壁细胞培养于无菌盖玻片上。
3.2 从培养基中移出盖玻片，吸除多余培养基。
3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。
3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟，使用荧光显微镜观察。
注：若需要用流式细胞仪检测，需将细胞用胰蛋白酶消化重悬后再进行染色。
保存条件
-20℃ 避光保存一年
注意事项
1.请根据实际情况调整 Propidium Iodide 工作液浓度与孵育时间。
2.只有死细胞的细胞核才会被染色红色。3. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。
3.Propidium Iodide 具有致癌性，为了您的安全和健康，请穿实验服并戴一次性手套操作。
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Propidium Iodide 相关抗体:
分子量
668.39
Formula
C27H34I2N4
CAS 号
25535-16-4
性状
固体
颜色
Pink to red
Emission (Em)
615
Excitation (Ex)
535
中文名称
碘化丙啶
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light
*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
溶解性数据
细胞实验：
DMSO 中的溶解度 : 100 mg/mL (149.61 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)
H2O 中的溶解度 : 3.57 mg/mL (5.34 mM; 超声助溶 (<60°C))
配制储备液
浓度
溶剂体积
质量
1 mg
5 mg
10 mg
1 mM
1.4961 mL
7.4807 mL
14.9613 mL
5 mM
0.2992 mL
1.4961 mL
2.9923 mL
10 mM
0.1496 mL
0.7481 mL
1.4961 mL
查看完整储备液配制表
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。
*
备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。
摩尔计算器
稀释计算器
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
质量
kg
g
mg
μg
ng
pg
=
浓度
M
mM
μM
nM
pM
×
体积
L
mL
μL
×
分子量 *
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
浓度 (start)
M
mM
μM
nM
pM
C1
×
体积 (start)
L
mL
μL
V1
=
浓度 (final)
M
mM
μM
nM
pM
C2
×
体积 (final)
L
mL
μL
V2
扫码获得动物溶解方案
动物溶解方案计算器
请输入动物实验的基本信息：
给药剂量
mg/kg
动物的平均体重
g
每只动物的给药体积
μL
动物数量
只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 :
mg/mL
纯度 & 产品资料
纯度: 99.69%
选择批次：
HY-D0815-40****
HY-D0815-84***
HY-D0815-10****
HY-D0815-24****
HY-D0815-70****
HY-D0815-10****
HY-D0815-35***
HY-D0815-61***
HY-D0815-32***
HY-D0815-52***
HY-D0815-10****
HY-D0815-25***
HY-D0815-40***
HY-D0815-28***
Data Sheet (655 KB)
SDS (419 KB)
COA (287 KB)
HNMR (261 KB)
RP-HPLC (244 KB)
LCMS (94 KB)
产品使用指南 (1538 KB)
染色示例
Description: Propidium Iodide is a cells dye with red fluorescence. Propidium Iodide only can be taken up by cells with impaired membrane integrity, it can be used in cell cycle and cell viability assays.
Method: For cell nucleus staining.
1. Cells are washed with PBS, and then incubates cells with Propidium Iodide (15 min).
2. Fluorescent images are acquired with a confocal laser scanning microscope (Olympus Fluoviewer. 3.1, Japan).
Description: Propidium Iodide is a cells dye with red fluorescence. Propidium Iodide only can be taken up by cells with impaired membrane integrity, it can be used in cell cycle, cell viability and necrocytosis assays.
Method: For cell walls staining.
Description: Propidium Iodide is a cells dye with red fluorescence. Propidium Iodide only can be taken up by cells with impaired membrane integrity, it can be used in cell cycle, cell viability and necrocytosis assays.
Method: For cell staining.
1. Fix cultured cells with paraformaldehyde (4%; 10 min).
2. Incubate cells with Propidium Iodide (50 mg/L; 15 min; dark).
3. Fluorescent images are acquired with a confocal laser scanning microscope.
参考文献
[1]. Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43(10):1031-8.
[Content Brief]
[2]. Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9.
[Content Brief]
[3]. A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9.
[Content Brief]